Figure 5. Representative 2% agarose gel of the restriction enzyme digestion analysis using MspI. The enzyme cut the PCR products into two fragments in unaffected males (I-4, II-20) and the normal female (II-4) but could not cut the products in affected males (II-10, III-22, III-23). Female carriers (I-3, II-6, II-19) exhibit a heterozygous status.