Table 1 of Hu, Mol Vis 2010; 16:1585-1592. Table 1 of Hu, Mol Vis 2010; 16:1585-1592.
Table 1. Primers used for candidate genes amplification related with congenital cataract and microcornea.
Exon |
Forward primer (5′-3′) |
Reverse primer (5′-3′) |
Product length (bp) |
Annealing temperature (°C) |
|---|---|---|---|---|
| CRYAA-1 | 5′-AGCAGCCTTCTTCATGAGC-3′ | 5′-CAAGACCAGAGTCCATCG-3′ | 584 | 60.6 |
| CRYAA-2 | 5′-GGCAGGTGACCGAAGCATC-3′ | 5′-GAAGGCATGGTGCAGGTG-3′ | 550 | 61.9 |
| CRYAA-3 | 5′-GCAGCTTCTCTGGCATGG-3′ | 5′-GGGAAGCAAAGGAAGACAGA-3′ | 511 | 60 |
| CRYBB1–1 | 5′-GGCAGAGGGAGAGCAGAGTG-3′ | 5′-CACTAGGCAGGAGAACTGGG-3′ | 380 | 58.3 |
| CRYBB1–2 | 5′-AGTGAGCAGCAGAGCCAGAA-3′ | 5′-GGTCAGTCACTGCCTTATGG-3′ | 301 | 61 |
| CRYBB1–3 | 5′-AAGCACAGAGTCAGACTGAAGT-3′ | 5′-CCCCTGTCTGAAGGGACCTG-3′ | 346 | 62.5 |
| CRYBB1–4 | 5′-GTACAGCTCTACTGGGATTG-3′ | 5′-ACTGATGATAAATAGCATGAACG-3′ | 400 | 58.3 |
| CRYBB1–5 | 5′-CGAGGAAGTCACATCCCAGT-3′ | 5′-CACAGAGCAGGAAGGGATA-3′' | 497 | 62.5 |
| CRYGC-1 | 5′-TGCATAAAATCCCCTTACCG-3′ | 5′-CCTCCCTGTAACCCACATTG-3′ | 514 | 59.2 |
| CRYGC-2 | 5′-TGGTTGGACAAATTCTGGAAG-3′ | 5′-CCCACCCCATTCACTTCTTA-3′ | 430 | 60.2 |
| CRYGD-1 | 5′-CAGCAGCCCTCCTGCTAT-3′ | 5′-GGGTCCTGACTTGAGGATGT-3′ | 550 | 60.3 |
| CRYGD-2 | 5′-GCTTTTCTTCTCTTTTTATTTCTGG-3′ | 5′-AAGAAAGACACAAGCAAATCAGT-3′ | 308 | 58.5 |
| GJA8–1 | 5′-CCGCGTTAGCAAAAACAGAT-3′ | 5′-CCTCCATGCGGACGTAGT-3′ | 420 | 56 |
| GJA8–2 | 5′-GCAGATCATCTTCGTCTCCA-3′ | 5′-GGCCACAGACAACATGAACA-3′ | 330 | 60 |
| GJA8–3 | 5′-CCACGGAGAAAACCATCTTC-3′ | 5′-GAGCGTAGGAAGGCAGTGTC-3′ | 350 | 58 |
| GJA8–4 | 5′-TCGAGGAGAAGATCAGCACA-3′ | 5′-GGCTGCTGGCTTTGCTTAG-3′ | 500 | 58 |
| MAF-1 | 5′-GTGGCGAGCATGGCTCTA-3′ | 5′-CCGCACTACCACCACCAC-3′ | 683 | 61.1 |
| MAF-2 | 5′-TACGCTGCGTTTGATCTTTG-3′ | 5′-AGGTGGTTCTCCATGACTGC-3′ | 224 | 61.4 |
| MAF-3 | 5′-GTGGTGGTGGTGGTAGTGC-3′ | 5′-CTCTCCTGCAGCCCATCTG-3′ | 611 | 65 |
| PAX6–4 | 5′-ACCTCGGTTGGGAGTTCAG-3′ | 5′-CGAAGTCCCAGAAAGACCAG-3′ | 161 | 54.6 |
| PAX6–5 | 5′-TGTGGTTGTCTCCTCCTCCT-3′ | 5′-GGGGTCCATAATTAGCATCG-3′ | 392 | 62.5 |
| PAX6–6-7 | 5′-AACGCCACTTTAAGCAAGGT-3′ | 5′-GGAGGGCAGATGTTCTCAA-3′ | 620 | 62.5 |
| PAX6–8 | 5′-AATCCACCCACTGTCCCG-3′ | 5′-CCAGCCACCTTCATACCG-3′ | 542 | 64.1 |
| PAX6–9 | 5′-TCAGGTAACTAACATCGCA-3′ | 5′-GTTGACTGTACTTGGAAGAA-3′ | 719 | 62.5 |
| PAX6–10 | 5′-AGGTGGGAACCAGTTTGATG-3′ | 5′-CATGGCAGCAGAGCATTTAG-3′ | 311 | 64.1 |
| PAX6–11–12 | 5′-TTCAGTCTGCTAAATGCTCTGC-3′ | 5′-AGTGCGAAAAGCTCTCAAGG-3′ | 592 | 62.5 |
| PAX6–13 | 5′-GAGGCTTGATACATAGGC-3′ | 5′-CCATAAGACCAGGAGATT-3′ | 452 | 64.1 |
| PAX6–14 | 5′-TTCCATGTCTGTTTCACAAAGG-3′ | 5′-GCCATTTTTCTTTCTTTCCTGA-3′ | 578 | 64.1 |