Molecular Vision: Goldstein, Mol Vis 2010; 16:1549-1569. Table 4

Table 4. Primer information and product sizes observed in crd3-affected and normal dogs, used to identify the canine crd3 mutation.

Primer pair	Forward primer name	Forward primer sequence	Forward primer location	Reverse Primer name	Reverse primer sequence	Reverse primer location	Observed size in normal (bp)	Observed size in affected (bp)
A. Initial sequencing of the region.
1	deletion_F1	CGAGGAAAAACCAACGAATGTGAT	Exon 14	deletion_R1	gagcaagcaatgaaaatagcagacc	Intron 14	521	521
2	deletion_F2	cctacctaataaccccatcccttgg	Intron 14	deletion_R2	tcagaaccctatccttttggcttg	Intron 14	627	627
3	deletion_F3	ctctgtgagtgaggtggacttagc	Intron 14	deletion_R3	ccaaggaatgaaacataaaatggaa	Intron 14	691	NO
4	deletion_F4	gagccagtagggagaggaacatca	Intron 14	deletion_R4	aaagacgcagaggaccacacaac	Intron 14	691	NO
5	deletion_F5	caagaatagtttgctaccttgtcagc	Intron 14	deletion_R5	ataccctggatcaactgccacatt	Intron 14	612	NO
6	deletion_F6	tgggtgttatgttcttcttgctca	Intron 15	deletion_R6	gaatgtgtagaggggcagagg	Intron 15	610	NO
7	deletion_F7	tcttagcagagccagagagccttc	Intron 15	deletion_R7	cagtttatccttccttcaacattcacg	Intron 15	622	NO
8	deletion_F8	atttggtggtcaacattcattggt	Intron 15	deletion_R8	cattcaaaacaactggaagcaggt	Intron 15	611	NO
9	deletion_F9	acgctgttgtgcagatcgtactgt	Intron 15	deletion_R9	aaccgtgagatgaaacatttgtgg	Intron 15	999	NO
10	deletion_F10	tctaaaatggaggaagtgtgaactaca	Intron 15	deletion_R10	ttcttggcttgggctaactct	Intron 15	602	NO
11	deletion_F11	tgtctgtccacgctctctgctatc	Intron 16	deletion_R11	tctctctacctctccctctgtttcca	Intron 16	688	NO
12	deletion_F12	ttgttacccgccataccccttgt	Intron 16	deletion_R12	ggagcaaagatgaaaaatacaggaa	Intron 16	759	NO
13	deletion_F13	ggaacaaacgcctctctcagtctt	Intron 16	deletion_R13	accagtagtccaaagggtccaggt	Intron 16	795	NO
14	deletion_F14	gaaatggtggggtggtagacaaga	Intron 16	deletion_R14	tactggggaaagatgagggttttt	Intron 16	774	NO
15	deletion_F15	tgactgtgaggggaagtgaagagtt	Intron 16	deletion_R15	cgttgaatgatggtatttggagatga	Intron 16	805	NO
16	deletion_F16	tgtcttctgttttggttgccagtg	Intron 16	deletion_R16	ccccgaactcatcccttactttct	Intron 16	835	NO
17	deletion_F17	acacatccccattccaactttcag	Intron 16	deletion_R17	accatcaactctcctggctctcag	Intron 16	796	796
18	deletion_F18	agcaccctcacaaacattcaga	Intron 16	deletion_R18	tcctctcaggcttttaccattatctt	Intron 16	798	798
19	deletion_F19	gaaaggaagtgtttgctgtagggaaa	Intron 16	deletion_R19	tctggatgaggtgagagtgaatgg	Intron 16	729	729
20	deletion_F20	aagattgaccgcttttcaccta	Intron 16	deletion_R20	ctacagatggctttgggcagtatg	Intron 16	718	718
21	deletion_F21	atccaggggaaatgaaaacaggag	Intron 16	deletion_R21	ggctgagagagcagaccagattgt	Intron 16	787	787
22	deletion_F22	tgagaagacaaatgaggggcactt	Intron 16	deletion_R22	tcaaaccaggcaatcaaacacctt	Intron 16	822	822
23	deletion_F23	cagaaggtgtttgattgcctggtt	Intron 16	deletion_R23	ttttgtttcccacagcatttttga	Intron 16	769	769
24	deletion_F24	tgctgatttctcccattattacca	Intron 16	deletion_R24	cacagttcctacaccaccaccaac	Intron 16	739	739
25	deletion_F25	aacttcatctaccctccttcacttg	Intron 16	deletion_R25	agtctcacctacctcactgggaat	Intron 17	752	752
B. Refined sequencing of the region.
26	deletion_F26	caagccaaaaggatagggttctga	Intron 14	deletion_R26	tcactccacaggtaaaaagccaaga	Intron 14	459	459
27	deletion_F27	tgactgaacccaggaagagagttg	Intron 14	deletion_R27	tgaatgaacaggcgaaaaagagag	Intron 14	462	462
28	deletion_F28	acctggattgggtttctttagg	Intron 14	deletion_R28	gcccgtggagtgggacataacta	Intron 14 and Intron 16	487	487
29	deletion_F29	ctggagcaatggggctggata	Intron 14	deletion_R29	aaaccaaaagcaataaataccacaa	Intron 14 and Intron 16	649	649
30	deletion_F30	atcagtcgttgagggtgacattga	Intron 14 and Intron 16	deletion_R30	ccgtggaaaagaaaaatcagacct	Intron 14 and Intron 16	421	421
31	deletion_F31	gggaaggatgggagaatgagagta	Intron 14 and Intron 16	deletion_R31	tcaaaggagcaatcggaaaagtct	Intron 16	476	476
32	deletion_F32	aaagggaaagggagggacagact	Intron 16	deletion_R32	tgtgagataaaggaaaataaagttgga	Intron 16	658	658
33	deletion_F33	cacaggctaacttttgctccatgt	Intron 16	deletion_R33	tgagtcttccttgccagtagaagc	Intron 16	479	479
34	deletion_F29	ctggagcaatggggctggata	Intron 14	deletion_R31	tcaaaggagcaatcggaaaagtct	Intron 16	NO	1,515