Figure 4. Effect of siRNA mediated AQP1 down-regulation (AQP1DR) on cell proliferation in HCEC cells. A: SRB (Sulforhodamine B) assay was used to determine the effect AQP1 down regulation (AQP1DR) on HCEC proliferation. AQP1DR decreased HCEC cell proliferation (significant at 3 and 5 days p<0.05, 95% CI as determined by the paired T-Test, [n=3]). B: Decrease in phosphorylated ERK 96 h post AQP1DR is shown by western blot analysis in HCEC cells.