Figure 2. Effective silencing of AQP1 and AQP5 using siRNA. Down-regulation of AQP1 expression in HCEC cells using siRNA as determined by (A) RT–PCR and (B) western blot analysis. Down-regulation of AQP5 expression in CEPI17 cells using siRNA as determined by (C) RT–PCR, (D) western blot analysis. Panel E shows down-regulation of AQP5 (green) in CEPI17 cells, 96 h post trasnfection with AQP5 siRNA. AQP5 down regulation was determined by Immunocytochemical analysis (DAPI was used to stain the nucleus).