Figure 4. Sub unit profile and protein cross-linking of soluble fraction of lens. A: Soluble protein was loaded onto a 12% polyacrylamide gel under reducing conditions. Molecular weight standards in kDa are indicated alongside of the gel. Lane 1- Molecular weight markers; Lane 2 - Group I; Lane 3 - Group II; Lane 4 - Group III; Lane 5 - Group IV. B: Densitometric quantification of cross links. Data are mean±SD (n=4). Intensity of protein bands above 31 kDa was quantified considering the intensity of Group I as 100%. Different superscripts above the bars denote that data are significantly different among the groups.