Table 2 of Renugadevi, Mol Vis 2010; 16:1514-1524. Table 2 of Renugadevi, Mol Vis 2010; 16:1514-1524.
Table 2. PCR primer sequences for TYR, P, TYRP1, MATP, and GPR143.
PCR primers |
PCR primer sequence (5’-3’) |
Melting temperature (°C) |
PCR product size (bp) |
|---|---|---|---|
| Tyrosinase (Tyr) gene [18,19] | |||
| Exon 1-1 | F-CAAACTGAAATTCAATAACATATAAG | 63 | 678 |
| R-GTGGACAGCATTCCTTCTCC | |||
| Exon 1-2 | F-TTCAGAGGATGAAAGCTTAAGATAAA | 62 | 521 |
| R-CGTCTCTCTGTGCAGTTTGG | |||
| Exon 1-3 | F-CTGGCCATTTCCCTAGAGC | 59 | 605 |
| R-CCACCGCAACAAGAAGAGTC | |||
| Exon 1-4 | F-CATCTTCGATTTGAGTGCCC | 60 | 514 |
| R-CCCTGCCTGAAGAAGTGATT | |||
| Exon 1-5 | F-CACCCATGTTTAACGACATCA | 59 | 225 |
| R-GCCAGTCCCAATATGGAATA | |||
| Exon 1-6 | F-GACTCTTCTTGTTGCGGTGG | 60 | 252 |
| R-CCCTGCCTGAAGAAGTGATT | |||
| Exon 2 | F-CCAACATTTCTGCCTTCTCC | 60 | 442 |
| R-TCAGCTAGGGTCATTGTCGAT | |||
| Exon 3 | F-AGTTATAAATCAAATGGGATAATC A | 60 | 296 |
| R-ACATTTGATAGGCACCCTCT | |||
| Exon 4 | F-CTGTTTCCAATTTAGTTTTATAC | 55 | 790 |
| R-TACAAAATGGCCTATGTTAAGC | |||
| Exon 5 | F-TGTCTACTCCAAAGGACTGT | 59 | 924 |
| R-GGCACTTAGCTGGATGTGTT | |||
| Pink eyed dilution (P) gene [20] | |||
| Exon 1 | F-GAGTTCTTACTTCGA | 52 | 172 |
| R-TAAACCCTCCCTGCCTGTTC | |||
| Exon 2 | F-GGTGCAAACGTTAGTCTCAG | 60 | 359 |
| R-CCAATCTGTGTGAAGTCCAC | |||
| Exon 3 | F-CTGGGAACACATACATTATT | 56 | 209 |
| R-GTGCAATGCTCAGAAACTCT | |||
| Exon 4 | F-AAGCTTGCTTTGTAGCCATT | 65 | 300 |
| R-CGATGTGCGGCCACCGCTGC | |||
| Exon 5 | F-GAAAAGTGTCTGAGTCTGGC | 62 | 177 |
| R-GTCCCGAAGGTGCCTGGGTT | |||
| Exon 6 | F-ATTTATACCTTACTGCTCTC | 58 | 183 |
| R-TTTCAGATCTCAGCCAGGCG | |||
| Exon 7 | F-GGACATGGGGTTTCTCCTGT | 59 | 264 |
| R-TGAGATGAAATGAGATTTCAC | |||
| Exon 8 | F-AGATCCCAGATGGTGTCTCA | 59 | 213 |
| R-AGGTCAGACTCCTTTAAACG | |||
| Exon 9 | F-AGAGGGAGGTCCCCTAACTG | 63 | 272 |
| R-ATCTCAAGCCTCCCTGACTG | |||
| Exon 10 | F-CTTTCGTGTGTGCTAACTCC | 57 | 195 |
| R-ACATCTTTGAGCTGACATCC | |||
| Exon 11 | F-GCAGCGCTTCATTAGGCTCA | 64 | 201 |
| R-GGCCAGAGAAGGCCCGGTTA | |||
| Exon 12 | F-GTCGTTTTAATATGGTGGCC | 56 | 256 |
| R-CCTGCAGAAGCAACCTTTA | |||
| Exon 13 | F-GCCTCTGTTCTACGAGCCTG | 63 | 231 |
| R-TGCCAGAACCTGGCCGCAA | |||
| Exon 14 | F-TTCACGATGTGTATAGTGGG | 59 | 239 |
| R-AAGTGGAGGTGTGCGTTTAC | |||
| Exon 15 | F-GATTACAGGCGTGAGCCACC | 61 | 293 |
| R-ACCCATCAACAGATACTTCC | |||
| Exon 16 | F-GAGGGTGTTGCTGATATCTG | 60 | 260 |
| R-GAATGTTCTGCTGCACACCA | |||
| Exon 17 | F-AGGCTCCAAGTCACAGACCG | 62 | 219 |
| R-CTTCTTGGAGAAGTGAATCAG | |||
| Exon 18 | F-AGTTGCGTAGGTTATGACAC | 58 | 235 |
| R-CCCATCCAGAATGTGACAAA | |||
| Exon 19 | F-GTTATGTATTTGCAGCCCCT | 58 | 197 |
| R-AATCCACCAAATACAATTGA | |||
| Exon 20 | F-GAATCGGTGTGTTAACAGTG | 59 | 266 |
| R-GTAGGCTTTCTTCATTCACC | |||
| Exon 21 | F-GCCTACCTTATGTTCACGTC | 58 | 212 |
| R-AATCAAAGAACAGTGGCTGG | |||
| Exon 22 | F-TGGTGGGTCTGACCCTAAGT | 59 | 219 |
| R-AGGCTATGTCCAGGCTAAAG | |||
| Exon 23 | F-ACAGTATGGCAGCTTCTCTG | 59 | 229 |
| R-ACTAACTGTTGCTTTGGGCT | |||
| Exon 24 | F-GAGAACAGAAGCTTACCACC | 55 | 204 |
| R-GCTTAGGAACTAGACAGTTTA | |||
| Exon 25 | F-CGTATCTCATGAGCTTATCC | 58 | 574 |
| R-AGCATACAATTTGAATGCTG | |||
| Tyrosinase-related protein 1 (TYRP1) gene [21] | |||
| Exon 1 | F-AGAAGTTCATCAGAGACATC | 50 | 189 |
| R-TCACCATCATTAATTACATT | |||
| Exon 2 | F-CGTGCTTCAGTCTTCTCTACA | 59 | 490 |
| R-GCAAGGACTTATGAACTCATTC | |||
| Exon 3 | F-CGCAAGGCAGATGTTTTCATG | 59 | 416 |
| R-AAGGCATCTTGTCTGTAAAGA | |||
| Exon 4 | F-AGACCAAACAGAAATGAATA | 47 | 305 |
| R-AAATTCTGACTCCAAGCTATC | |||
| Exon 5 | F-AAAGAGCGACAATAAGAACTC | 50 | 319 |
| R-AAAGCCTTCTCAAAGAAACTT | |||
| Exon 6 | F-TTGCTATTACCTGGAAAAGTG | 51 | 275 |
| R-TGCAAAAAGCATATGAAAATG | |||
| Exon 7 | F-ATACGTTGTCTTTGGAATAAT | 51 | 252 |
| R-ATACCGTGATTACTCTACTTG | |||
| Exon 8 | F-TGTCCACTTTTTGGTGATAAC | 50 | 323 |
| R-ATTCAACCAGGTGGTTTTGTG | |||
| Membrane-associated transporter protein (MATP) gene [22] | |||
| Exon 1-1 | F-AGGCTCCACGTCAAATCCAG | 63 | 260 |
| R-GGTCACATACGCTGCCTCCA | |||
| Exon 1-2 | F-CAGACTCATCATGCACAGCA | 58 | 252 |
| R-ATGCCCACGAGCATCATGAC | |||
| Exon 1-3 | F-CAGCATTGTGTGGTTCCTCA | 58 | 261 |
| R-GGTCAAACACATGAACATCCTC | |||
| Exon 2 | F-AACGCGGATGATTCTAAAACAGGA | 65 | 280 |
| R-CTCATTGTCTGGGGAGCTGA | |||
| Exon 3-1 | F-GGGAGTGTCTATGCATGAGG | 65 | 324 |
| R-GATAGAACCATACTCGTACATTCC | |||
| Exon 3-2 | F-GCCCCACTTACAGAGGTTGC | 63 | 224 |
| R-CAACAAAGAGCAAGAATATTTTCCCTTG | |||
| Exon 4 | F-AGCTGGCTGAGTTTCTGCAG | 62 | 265 |
| R-CCTCAACAGGTGTTAATGGAGG | |||
| Exon 5 | F-AGAGGTGGAGAAGCAGAGTG | 64 | 236 |
| R-GAAGACATCCTTAGGAGAGAG | |||
| Exon 6 | F-ATGAGGCACTGCCAGCTGTA | 64 | 286 |
| R-CCCAAGGCAGAGGTTCAATG | |||
| Exon 7 | F-GCCCTAAATGACAGTTCCTTG | 58 | 326 |
| R-TGTGCTTCACTGTCTCTGAG | |||
| G protein-coupled receptor 143 (GPR143) gene [23] | |||
| Exon 1-1 | F-GAGCCTGGCTCTACTGCAGGCGCT | 64 | 250 |
| R-TGCCCAGGCAGAGCGCGTGGAAGG | |||
| Exon 1-2 | F-AGCCACGCAGCTCGTGCTGAGCTTCCAGCC | 68 | 250 |
| R-CCCAGGCGCTGATCAGATTCCAACCCGCG | |||
| Exon 2 | F-TCATTTTTCCAAAGCAAGAAGTCAGC | 66 | 293 |
| R-GCAGGACGTGAGAACCTGCATT | |||
| Exon 3 | F-GTCTACCCTGCCGTCTCAAGGATG | 66 | 248 |
| R-CGCTCAGTGCCATCTCTTATCTTCC | |||
| Exon 4 | F-GTTCCAGGCAGGCCTCTGTGC | 68 | 229 |
| R-GGCTCATGTATTCCCTGCAAGACAAC | |||
| Exon 5 | F-TTTCCCTTTTTGTTCTCATCCTCTTA | 63 | 299 |
| R-AGGACAACATGTGTCACTGTCTGAG | |||
| Exon 6 | F-ACCTGCTTCCATTGCCTTCTCTGTC | 68 | 288 |
| R-CTTCCCTTTGGAACTTCTGGTCACG | |||
| Exon 7 | F-GAAATTCTTCTCTGACTCTCCAGCATT | 63 | 278 |
| R-TGACAGAGTGAGACCTTGTCTCTGA | |||
| Exon 8 | F-ATGGTCCCTTCCAAGCGAGTCC | 68 | 492 |
| R-TCACATGAGAGGTGCTGCTGAACAC | |||
| Exon 9 | F-TGAAAAACTCCATGCACTGAATACT | 61 | 597 |
| R-TGCATAACTGTACATGTATTTATTTTCTTTTG | |||