Figure 8. Typical images of DAPI-stained paraformaldehyde-fixed sections from lenses unirradiated control lenses and lenses from mice X-irradiated at 3-month of age. Panel A was from unirradiated 3-month controls, panel B was from 14 month unirradiated controls, panel C was from 14-month X-irradiated lens, and panel D was from a 26-month old unirradiated control. Each image was derived from a panorama taken with a 10× objective (see Methods). The dashed lines show the regions of the lenses used for counting DNA fragments in different regions of cortex (see Figure 9A,B). These include both bow regions, and the cortex below the central zone. The posterior cortex was included if nuclear fragments were present. The non-lens portions of the eye have been deleted for clarity. Original magnification was 200×.