Figure 7. A comparison of nuclei and nuclear cortical fragments in a typical old-mouse eye section using both DAPI and H&E stains. DAPI (A) and H&E (B) staining are compared. All of the included nuclei stained with DAPI (white) were also stained blue by Hematoxylin. All other H&E and DAPI images demonstrated the same correspondence (not shown), but DAPI was much easier to score at lower magnification so was generally used in counting nuclei and nuclear fragments. Original magnification was 200×.