Figure 12. The number of 8-OH-dG positive cortical nuclear fragments found beneath the central zone. Fixed lens sections were stained with antibodies to 8-OH-dG (green) and Dapi (Red) as described in the methods (see also figure 11). The number of 8-OH-dG positive nuclear fragments resistant to 2N HCl lying only in the cortex below the Central Zone were counted and compared. The 8-OH-dG positive fragments (green) increased with age and were significantly higher in the X-irradiated lenses at 12.5 months and 14 months. Five to 7 of samples were analyzed at each age, except the 3-month X-ray with 2 mice lenses; and the 7-month X-ray with 4.