Figure 11. Typical panoramas of fixed-eye lens sections stained with antibodies to 8-OH-G lesions and also stained for total DNA. Fixed-eye lens sections were prepared and stained with antibodies to 8-OH guanosine and 8-OH deoxyguanosine (green), and Dapi (red) as described in the Methods. The sections were treated with 2N HCl to remove 8-OH G in RNA leaving only 8-OH deoxyguanosine residues. Head-only X-irradiation was at 3 months. The non-lens parts of the eye have been blacked out for clarity. Original magnification was 200×.