Figure 10. Fixed-eye mouse lens sections were stained with; antibodies to alpha smooth muscle actin shown in green, and aquaporin 0 shown in red and also DNA shown in blue as described in the Methods. Typical staining of lens fibers with aquaporin O antibodies (red) and myofibroblast-like cells (green) are shown. Note that aquaporin O comprises approximately 60% of lens fiber membrane proteins, and that myofibroblasts are normally not present in the normal young mouse lens (see text). A: Posterior of 14-month X-irradiated lens, arrows point out myofibroblasts (green). B: Posterior region of typical old (26-month) mouse lens lacking myofibroblasts. Panels C-G show images of the bow regions of irradiated or unirradiated control lenses of various ages that were all negative for myofibroblasts. Original magnification was 200×.