Figure 5. Expression of miR-9 increases in ARPE-19 cells following treatment with 5-aza-2’-deoxycytidine. Cells were treated with 1 μM 5-aza-2’-deoxycytidine for 3 days, and miR-9 expression was estimated using real time RT–PCR. The expression of TGM2, a gene known to be upregulated by this treatment, was also analyzed. A: 5-aza-2’-deoxycytidine increased miR-9 expression. B: 5-aza-2’-deoxycytidine increased TGM2 expression. *p<0.05 compared to control, n=4.