Figure 7 of Hu, Mol Vis 2010; 16:1415-1428.


Figure 7. Inner limiting membranes of protein O-mannose N-acetylglucosaminyltransferase 1 (POMGnT1) knockout and Largemyd mice exhibit reduced elasticity. Flatmounted inner limiting membranes from postnatal day 2 (P2) newborn mice were immunostained with anti-laminin. AFM indentation experiments were performed to determine the Young’s modulus. Micrographs of laminin immunofluorescence staining of wild type (A), protein O-mannose N-acetylglucosaminyltransferase 1 knockout (B) and Largemyd mice (C) are shown. Note that the mutant inner limiting membranes showed many holes (B and C). D and E are representative force displacement curves for indentation experiments on the inner limiting membranes of protein O-mannose N-acetylglucosaminyltransferase 1 knockout and Largemyd mice. Apparent Young’s modulus measurements from inner limiting membranes isolated from 4 mice for each genotype are shown in F. The bars represent means with standard deviations (p<0.01, ANOVA). When compared to the controls, significantly reduced Young’s modulus was observed for both POMGnT1 knockout (p<0.01) and Largemyd (p<0.01) inner limiting membranes (post hoc Student’s t-test). Asterisks in F indicate p<0.01. These results indicated that the inner limiting membranes of protein O-mannose N-acetylglucosaminyltransferase 1 knockout and Largemyd mice exhibited reduced elasticity compared to the wild type. MPa represents megapascal. nN represents nanonewton. The scale bar in C is equal to 25 μm.