Figure 1 of Hu, Mol Vis 2010; 16:1415-1428.

Figure 1. Dystroglycan distribution at the Müller glia endfeet and inner limiting membrane are disrupted in protein O-mannose N-acetylglucosaminyltransferase 1 (POMGnT1) knockout retina. Adult retinal sections were double stained with anti-β-dystroglycan (green fluorescence) and anti-laminin (red fluorescence). Wild type (A-C) and knockout (D-F) retinas were immunostained with anti β-dystroglycan (A, D) and laminin (B, E). All sections were counterstained with 4',6-diamidino-2-phenylindole to show nuclei. Merged image of A and B is shown in C. Merged image of D and E is shown in F. Note β-dystroglycan and laminin staining at the inner limiting membrane had breaks (arrows in D and E), indicating a lack of Müller glia endfeet and inner limiting membrane at these locations. Abbreviations: DG represents dystroglycan; ILM represents inner limiting membrane; INL represents inner nuclear layer; IPL represents inner plexiform layer; ONL represents outer nuclear layer; OPL represents outer plexiform layer, DAPI represents 4',6-diamidino-2-phenylindole; +/+ represents wild type; −/− represents homozygous knockout. Scale bar in F is equal to 50 μm.