Figure 7. Western blotting analysis for
evaluating the effects of p62 or hsp70 RNA interference
on LC3 levels in ARPE-19 cells treated with bafilomycin. A: The
cells were exposed simultaneously to 50 nM bafilomycin and the
transfection reagent, nonsilencing RNA, hsp70 siRNA, or p62
siRNA for 24 h. The untreated control is represented by 0, while tr.
stands for transfection reagent, negative for nonsilencing siRNA, hsp70,
p62 for silencing siRNAs. α-tubulin staining was used to
ensure equal loading of proteins. B: Normalization against
α-tubulin. C: The ratio of a lipidated LC3-II to a nonlipidated
LC3-I. Experiments were repeated two independent times.