Figure 5. Analysis of perinuclear aggregates. A: Transmission electron micrographs of control ARPE-19 cells (blank), cells exposed to transfection reagent, and cells exposed to 5 µM MG-132 for 24 h with or without transfection reagent, nonsilencing siRNA, hsp70 siRNA, or p62 siRNA (all 30 nM). The scale bar equal to 2 μm. B: Transmission electron micrographs of the cells simultaneously treated with 5 µM MG-132 and the transfection reagent, nonsilencing RNA, hsp70 siRNA, or p62 siRNA for 24 h, and then allowed to recover in normal cell culture medium for 24 or 48 h. Arrows indicate autophagosomal structures.