Figure 1. Western blotting analysis for evaluating p62 RNA interference efficacy and measuring its effects on ubiquitin levels in ARPE-19 cells. Three different p62 siRNAs were used with three different concentrations (30, 50, and 80 nM). The samples of p62 siRNA were also treated with 5 µM MG-132. Bl. stands for untreated control, tr. for transfection reagent (with or without 5 µM MG-132), and negative siRNA for nonsilencing siRNA (with or without 5 µM MG-132). All exposures lasted 24 h. α-Tubulin staining was used to ensure the equal loading of proteins.