Figure 4. Expression of vasohibin-1 protein within mouse cornea, as detected by western blot analysis. Mice were administered with subconjunctival injection of Ad-Vasohibin-1 at 10⁹ viral particles. Corneas were harvested at different time points, and protein samples were extracted. Ten corneal samples were pooled together for each western blot analysis. A mouse antihuman vasohibin-1 monoclonal antibody was used for the western blot. No vasohibin-1 western blot signal was detected before Ad-Vasohibin-1 was injected. A relatively weak signal was detected on day 5 and was maintained at high levels until day 14 after injection.