Figure 5. NPHP1 and NPHP4 interact independently of RPGR. A: Bovine retinal lysates were subjected to IP using anti-RPGR antibody followed by immunoblotting of the precipitated proteins and supernatant (sup). B, C: RPGR-immunodepleted RPGR supernatant (RPGR-ID sup) was immunoprecipitated with either anti-NPHP4 (B) or anti-NPHP1 (C) antibodies and analyzed by SDS–PAGE and immunoblotting. Lanes are indicated. Molecular weight markers in kDa are shown on the left.