Figure 4. Immunodepletion of NPHP1 in bovine retinas. A: NPHP1-containing complexes were immunodepleted from bovine retinal lysates, as described above. The supernatant (sup) was subsequently used for IP experiments. B: NPHP1-immunodepleted supernatant (NPHP1-ID sup) was subjected to IP with anti-RPGR antibody followed by immunoblot analysis. Lanes are indicated. Molecular weight markers in kDa are shown on the left.