Figure 6 of Lewis, Mol Vis 2010; 16:1361-1372.

Figure 6. Laser scanning confocal images of radial sections (A, B) and wholemounts (C- F) of control rabbit retina labeled with anti-bromodeoxyuridine (BrdU; red), anti-vimentin (green), and anti-glial fibrillary acidic protein (GFAP; blue) from eyes injected with 5-fluorouracil (5-FU). The anti-BrdU detects the 5-FU, which is present in all Müller cell nuclei and illustrates that the notch is visible in most of them (A-F). In addition, GFAP filaments are present in only some of the notches (C, D), while vimentin filaments appear to be present in all of the notches (E, F). The images in the right panels are the same as those on the left but illustrate only the BrdU labeling. The insets in B are higher magnifications of the regions marked with the brackets to more easily visualize the notch (arrows). Abbreviations: GCL represents ganglion cell layer; INL represents inner nuclear layer; ONL represents outer nuclear layer. The scale bars are equal to 20 µm.