Figure 2. Laser scanning confocal images of rabbit retinas detached for 4 days labeled with anti-bromodeoxyuridine (BrdU; red) and anti-vimentin (green). The z-stack of images is projected in three dimensions at varying contrasts, illustrating that the BrdU-labeled nuclei appear to be associated with only one Müller cell process. A shows two nuclei lined up directly with a single Müller cell “stalk” of intermediate filaments (arrows). The image in B is rotated slightly so that the lower nucleus in the INL appears off to the side of the major intermediate filament bundle. Abbreviations: GCL represents ganglion cell layer; IPL represents inner plexiform layer; INL represents inner nuclear layer; ONL represents outer nuclear layer; OLM represents outer limiting membrane. The scale bar is equal to 20 µm.