Table 1 of Shyam, Mol Vis 2010; 16:122-129. Table 1 of Shyam, Mol Vis 2010; 16:122-129.
Table 1. Primer pair sequences, annealing temperature, and product sizes of Wnt-related genes.
Genes examined |
Forward primer |
Reverse primer |
Annealing temperature (°C) |
Expected product size (bp) |
|---|---|---|---|---|
| Wnt1 | 5’-TCCTCCACGAACCTGCTTAC-3’ | 5’-GCCTCGTTGTTGTGAAGGTT-3’ | 54 | 491 |
| Wnt2 | 5’-GGTGATGTGCGATAATGTGC-3’ | 5’-GCCAGCTCTGTTGTTGTGAA-3’ | 52 | 404 |
| Wnt2b | 5’-AAGATGGTGCCAACTTCACC-3’ | 5’-GGCCACAGCACATGATTTCAC-3’ | 54 | 188 |
| Wnt3 | 5’-CTGTGAGGTGAAGACCTGCTG-3’ | 5’-GATGCAGTGGCATTTTTCCT-3’ | 54 | 357 |
| Wnt3a | 5’-GGTGGCTGTAGCGAGGACAT-3’ | 5’-ATGCCGTGCGAGCTGACGTT-3’ | 56 | 454 |
| Wnt4 | 5’-GCATCTCAGAGGAGGAGACG-3’ | 5’-TCAGAGCATCCTGACCACTG-3’ | 56 | 363 |
| Wnt5a | 5’-ATTTTTCTCCTTCGCCCAGGT-3’ | 5’-GGCTCATGGCGTTCACCAC-3’ | 55 | 358 |
| Wnt5b | 5’-CCAAAGGATCAGAGGAGCAG-3’ | 5’-TACACCTGACGAAGCAGCAC-3’ | 56 | 483 |
| Wnt6 | 5’-TGGTTATGGACCCTACCAGCA-3’ | 5’-CGTCCATAAAGAGCCTCGAC-3’ | 54 | 467 |
| Wnt7a | 5’-CTGGAGGAGAACATGAAGC-3’ | 5’-ACAGCACATGAGGTCACAGC-3’ | 54 | 354 |
| Wnt8a | 5’-TCCCAAGGCCTATCTGACCTAC-3’ | 5’-CCGGCCCTGTTGTTGTGA-3’ | 57 | 407 |
| Wnt10b | 5’-AATGCGAATCCACAACAACA-3’ | 5’-ACAGCACATAGCAGCACCAG-3’ | 54 | 448 |
| Wnt11 | 5’-CTACACAACAGTGAAGTG-3’ | 5’-CCCACCTTCTCATTCTTCATGC-3’ | 55 | 298 |
| Dsh1 | 5’-CACCCTGAACCTCAACAGTGG-3’ | 5’-CCCTTCACTCTGCTGACTCC-3’ | 56 | 201 |
| Dsh2 | 5’-CCTTCAGCAGCGTCACAGATTCC-3’ | 5’-AGTGGGCAGCAGGGGCC-3’ | 59 | 895 |
| Dsh3 | 5’-CAGCCCCCTTCTGTGCTGATAA-3’ | 5’-AGAAGGTGATCTTGTTGA-3’ | 59 | 1196 |
| Fzd1 | 5’-TGCGAGGCGCTCATGAACAA-3’ | 5’-CCTCGGCGAACTTGTCATTA-3’ | 54 | 570 |
| Fzd2 | 5’-CGTCCTCAAGGTGCCATCCTA-3’ | 5’-CAGCCCGACAGAAAAATGAT-3’ | 54 | 248 |
| Fzd4 | 5’-CTCGGCTACAACGTGACCAAGAT-3’ | 5’-AATATGATGGGGCGCTCAGGGTA-3’ | 57 | 604 |
| Fzd5 | 5’-CTGCTACCAGCCGTCCTTCAGT-3’ | 5’-CCATGCCGAAGAAGTAGACCA-3’ | 59 | 319 |
| Fzd7 | 5’-CGACGCTCTTTACCGTTCTC-3’ | 5’-GCCATGCCGAAGAAGTAGAG-3' | 54 | 246 |
| Fzd8 | 5’-GGACTACAACCGCACCGACCT-3’ | 5’-ACCACAGGCCGATCCAGAAGAC-3’ | 59 | 406 |
| LRP5 | 5’-AAGATCATTGTGGACTCGGAC-3’ | 5’-GAAAGGCTCGCTTGGG-3’ | 52 | 395 |
| LRP6 | 5’-ACTGTATCCCTGTGGCTTGG-3’ | 5’-CCCTTCATACGTGGACACA-3’ | 54 | 426 |
| CTNNB1 | 5’-CATGGAACCAGACAGAAAAGC-3’ | 5’-GCTACTTGTTCTTGAGTGAAG-3’ | 52 | 200 |
| GSK3β | 5’-GCAGCAGCCTTCAGCTTTTGG-3’ | 5’-CCGGAACATAGTCCAGCACCAG-3’ | 59 | 358 |
| APC | 5’-TCCACAACATCATTCACTCACAG-3’ | 5’-TGCTCGCCAAGACAAATTCC-3’ | 53 | 505 |
| TCF1 | 5’-GAGCAAAGAGGCACTGATCC-3’ | 5’-CTGGTTGAGGCCAGTGGTAT-3’ | 54 | 361 |
| TCF3 | 5’-GGGTCTTCCATCCTCGGTGTA-3’ | 5’-GAGTAGATCGAGGCCAGTGC-3’ | 56 | 488 |
| TCF4 | 5’-GTTTGTATTTTTTGGCG-3’ | 5’-GAATGGCTGCCTTAGGG-3’ | 49 | 467 |
| TCF7 | 5’-GAGCCAAGGTCATTGCAGAGT-3’ | 5’-GTGGTGGATTCTTGGTGCTT-3’ | 54 | 222 |
| Dkk1 | 5’-GATCTGTAAACCTGTCCT-3’ | 5’-GAAGAATTACTGGCTTGATG-3’ | 50 | 149 |
| Dkk2 | 5’-CTGATGGTGGAGAGCTCACAG-3’ | 5’-ATTATTGCAGCGGGTACTGG-3’ | 56 | 311 |
| sFRP1 | 5’-CAACCTGCTGGAGCACGAGAC-3’ | 5’-CGCTGGCACAGAGATGTTCA-3’ | 58 | 379 |
| sFRP2 | 5’-CACGGCATCGAATACCAGAAC-3’ | 5’-GATGCAAAGGTCGTTGTCCT-3’ | 58 | 308 |
| sFRP3 | 5’-GGGCTGTGAGCCCATACTCAT-3’ | 5’-GGCAGCCAGAGCTGGTATAG-3’ | 57 | 379 |