Figure 3. Actin staining in human trabecular meshwork cells. Cells were treated with 1 mM H₂O₂ for 30 min and were fixed immediately after (0 h) or 4 h later (4 h), and stained with Alexa Fluor 488-phalloidin. Cells untreated were used as controls. Results showed a significant reduction in actin stress fibers upon H₂O₂ treatment, a previously documented cell response [22] to oxidative stress. Scale bar represents 20 µm.