Figure 2. β-catenin and secreted
frizzled-related protein 1 (sFRP1) protein levels in human trabecular
meshwork (TM) cells.
β-catenin (A) and sFRP1 (B) protein levels were assayed
in human TM cells. Cells were treated with 1 mM H2O2
for 30 min. Lysates were harvested 0, 1, 2, or 4 h later. Control cells
(C) were left untreated. Protein levels are expressed as ratios
relative to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
All experiments were conducted in at least three different cell lines
from three different donors. Data from one representative experiment