Figure 3. Retinal ganglion cell (RGC) survival in glaucomatous DBA/2J mice following transfection with adeno-associated virus serotype 2-cytomegalovirus-green fluorescent protein (AAV2-CMV-GFP) or adeno-associated virus serotype 2-wild type (AAV2-WT) optic atrophy type 1 (mOPA1). A, B: The OPA1 antibody recognized 90-kDa (L) and 80-kDa (S) isoforms of OPA1 protein in the total retinal protein extracts of glaucomatous DBA/2J mice transfected with AAV2-CMV-GFP. Conversely, AAV2-WT mOPA1-trasfected glaucomatous mice had significantly increased isoforms of the OPA1 protein compared with glaucomatous DBA/2J mice treated with AAV2-CMV-GFP (n=4 retinas/group). Data represent the mean±SD *Significant at p<0.05 compared with nine-month-old glaucomatous DBA/2J mice transfected with AAV2 Null. C: OPA1 immunoreactivity in glaucomatous DBA/2J mice transfected with AAV2 Null. D: OPA1 immunoreactivity in glaucomatous DBA/2J mice transfected with AAV2-CMV-WT mOPA1. Abbreviations: ONL represents outer nuclear layer; OPL represents outer nuclear layer; IPL represents inner plexiform layer; GCL represents ganglion cell layer; CA represents central artery. Scale bar indicates 20 µm (C, D).