Figure 2. Effect of subconjunctival injection of OVA-gold in the conjunctiva. OVA-gold, gold or PBS (10 μl) was injected into the subconjunctival
space of DO11.10 mice. Twenty-four hours later, conjunctivas were collected for immunohistochemical analyses. MBP-positive
eosinophils and CD4-positive T cells were detected in the OVA-gold-injected group (A). Control samples were not stained with any primary antibodies. MBP-positive eosinophils were quantified in the OVA-gold
and control groups (B). The double asterisk indicates a p<0.01 compared to PBS and the gold groups.