Figure 1. In vitro antigenicity of OVA-gold. Splenocytes from naïve DO11.10 mice were cultured in vitro with OVA, gold, or OVA-gold. OVA-gold was added to the culture at a concentration of 1, 10, and 100 μg/ml of OVA . Gold alone was added to the culture so that the same amount of gold per well was added as in the OVA-gold wells. Proliferation was evaluated by [³H]thymidine incorporation. Background cpm (no Ag) was 1707+/−290. One representative data of two experiments is shown. The double asterisk indicates a p<0.01 compared to the background.