Table 1 of Wiley, Mol Vis 2010; 16:1253-1259. Table 1 of Wiley, Mol Vis 2010; 16:1253-1259.
Table 1. Comparison of the steps required for BrdU and EdU labeling of lens explants or whole lenses.
| Step | BrdU/Explant | Time | EdU/Whole | Time |
|---|---|---|---|---|
| 1 | Inject BrdU, wait for incorporation | 1 h | Inject EdU, wait for incorporation | 1 h |
| 2 | Isolation of lenses/preparation of explants | 2–4 h | Isolation/cleaning lenses | 30 min |
| 3 | Fixation | 1 h | Fixation | 10 min |
| 4 | Wash | 30 min | Wash | 10 min |
| 5 | HCl Denature | 1 h | Permeabilization | 1 h |
| 6 | Wash | 1 h | Wash | 10 min |
| 7 | Block | 1–2 h | “Click” reaction/ DRAQ-5 staining | 1 h |
| 8 | Primary Antibody | ~12 h | Wash | 1 h |
| 9 | Wash | 1 h | Ready to View | |
| 10 | Secondary Antibody plus nuclear stain | 2 h | ||
| 11 | Wash | 1 h | ||
| 12 | Ready to view | |||
| Total | ~23–26 h (2–3 laboratory days) | ~5 h (one laboratory day) |