Figure 4. Validation of micro array data by semiquantitative RT–PCR. Reverse transcription polymerase chain reaction analysis of the selected differentially expressed genes. Ribosomal protein large 35 (RPL35) was served as an internal control. Abbreviations: LEC – limbal epithelial cells, MC-L – mesenchymal cells of limbus, MSC-BM – mesenchymal stem cells of bone marrow, -RT – no reverse trasncriptase, CDH1 – cadherin 1 (E-cadherin), COLVIA1 – collagen 6 alpha 1, COL4A2 – collagen 4 alpha 2, IL-1B – interleukin 1 beta, FN1 – fibronectin 1, MAL2 - T-cell differentiated antigen 2, CTGF – Connective tissue growth factor, FGFR1 – fibroblast growth factor receptor 1, BDNF – brain derived nerve growth factor, CCL2 – chemokine ligand 2, CHI3L1 – chitanase 3 like 1, MMP2 – matrix metallo peptidase 2, IL1A – interleukin 1 alpha, KRT7- cytokeratin 7, DCN – decorin, Ang 1 – angiopoientin, bFGF – basic fibroblast growth factor, NTRK2 – neurotrophin tyrosine kinase receptor 2.