Figure 2. Characterization of limbal explant culture derived epithelial (LEC) and mesenchymal like cells (MC-L). Following immunoctytochemical analysis (A-L) epithelial cells showing positive for ABCG2 (A), CK3/CK12 (B; green fluorescence), CK19 (C; red fluorescence), CK14 (D), E-Cadherin (E), vimentin (F; green fluroscence), double immunostaining for PAX-6 (green fluorescence) and vimentin (red fluorescence; G). Mesenchymal like cells of limbus (H-L) showing positive for vimentin (H) and negative for cytokeratin 3/12 (I), cytokeratin 14(J), CD34 (K), and nestin (L). Nuclear staining was performed with propidium iodide (red; A, B, D, E, H-L). Scale bar=20 µm (A-C, E-H, and J) and 10µm (D, I, K, L). Flow cytometry analysis (M-V) was performed by incubation of the mesenchymal like cells of limbus with the indicated antibodies. M: Isotype controls for FITC and PE, N: CD90 FITC and CD44 PE, O: CD40-FITC and HLA-ABC PE, P: CD11b FITC and HLA-ABC PE, Q: CD34 FITC and CD10 PE R: Isotype control for FITC and APC, S: CXCR4 APC and CD90 FITC, T: CD40L APC and CD40 FITC, U: Isotype control for FITC and PerCP, V: CD138 PerCP and CD13 FITC.