Figure 3. Multiple amino-acid sequence alignment of CYP1B1 from different species. Sequence alignment was generated by ClustalW. The positions of mutated amino-acids newly reported in this study are indicated by arrows and red letters. The COOH-terminal amino-acids of C-helix, NH₂-terminal amino-acids of D-helix and heme binding loop (HBL) are indicated below the sequence alignment, by a line.