Figure 2. Detection of three novel CYP1B1
mutations in Moroccan PCG families by direct DNA sequencing. Sequencing
results of probands from families PCG-101 (A), PCG-100, and
PCG-64 (B) and PCG-84 (C). Chromatogram of a heterozygous
subject for g.4330–4331delG is shown in C. The names of the
mutations and their corresponding amino-acid change are indicated above
each chromatogram. Control sequences are shown for comparison purposes.
Arrows indicate the changed nucleotides and curley bracket the deleted
nucleotides in the g.4330–4331delTG allele. Because of the presence of
a three GT repeat in this region, it is not possible to determine
exactly which of the positions is deleted. Therefore, the position of
this deletion was arbitrarily indicated. Htr: heterozygous mutation and
Hmz: homozygous mutation.