Figure 2. Quantification of retinal
pigment epithelium-(RPE) and photoreceptor-specific gene expression.
Quantitative real-time-polymerase chain reaction (QRT–PCR) was
performed, comparing retina–RPE samples from intact mice and those
grown in organotypic cultures starting from postnatal day 7. Analyzed
were expression levels of Rpe65 (the rate-limiting enzyme required to
generate 11-cis retinal), one rod-specific gene (rhodopsin), and
three cone-specific genes (ultraviolet [UV] and middle-wavelength [MWL]
opsin and cone transducin [Gnat2]). Explants contain 2-fold to
3-fold less mRNA for the tested genes at P7+11DIV than age-matched
(P18) tissues that developed in vivo. Data are represented as
mean±standard deviation of three different sets of experiments.