Table 3 of Krishnan, Mol Vis 2010; 16:1169-1177.


Table 3. Signal transduction pathway gene profile supporting the expansion of serum-free B27 supplemented limbus/corneal culture (serum-dependent culture as control).

Symbol Limbus Cornea Description Gene Name
Mitogenic Pathway
EGR1 12.06 1.16 Early growth response 1 AT225/G0S30
FOS 67.78 1.6 V-fos FBJ murine osteosarcoma viral oncogene homolog AP-1/C-FOS
JUN 8.51 1.64 Jun oncogene AP-1/AP1
Wnt Pathway
CCND1 4.28 −1.72 Cyclin D1 BCL1/D11S287E
JUN 8.51 1.64 Jun oncogene AP-1/AP1
LEF1 12.06 1.13 Lymphoid enhancer-binding factor 1 DKFZp586H0919/TCF1ALPHA
MYC 4.28 −3.56 V-myc myelocytomatosis viral oncogene homolog (avian) MRTL/bHLHe39
PPARG 2.14 −1.33 Peroxisome proliferator-activated receptor gamma CIMT1/NR1C3
TCF7 12.06 1.13 Transcription factor 7 (T-cell specific, HMG-box) TCF-1
VEGFA 1.07 −1.74 Vascular endothelial growth factor A MVCD1/VEGF
WISP1 11.65 1.13 WNT1 inducible signaling pathway protein 1 CCN4/WISP1c
Hedgehog Pathway
BMP2 8.52 −3.57 Bone morphogenetic protein 2 BMP2A
BMP4 4.32 −2.46 Bone morphogenetic protein 4 BMP2B/BMP2B1
EN1 11.76 1.13 Engrailed homeobox 1 Engrailed 1
FOXA2 12.06 1.13 Forkhead box A2 HNF3B/TCF3B
PTCH1 2.01 −2.46 Patched homolog 1 (Drosophila) BCNS/HPE7
WNT1 12.06 1.13 Wingless-type MMTV integration site family, member 1 INT1
WNT2 12.06 1.17 Wingless-type MMTV integration site family member 2 INT1L1/IRP
TGF-Beta Pathway
CDKN1A 3.00 −2.47 Cyclin-dependent kinase inhibitor 1A (p21, Cip1) CAP20/CDKN1
CDKN1B 5.99 1.65 Cyclin-dependent kinase inhibitor 1B (p27, Kip1) CDKN4/KIP1
CDKN2A −1.34 −6.95 Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) ARF/CDK4I
CDKN2B 2.12 −1.75 Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) CDK4I/INK4B
Survival Pathway
PI3 Kinase/AKT Pathway
BCL2 12.06 1.13 B-cell CLL/lymphoma 2 Bcl-2
CCND1 4.28 −1.72 Cyclin D1 BCL1/D11S287E
JUN 8.51 1.64 Jun oncogene AP-1/AP1
MYC 4.28 −3.56 V-myc myelocytomatosis viral oncogene homolog (avian) MRTL/bHLHe39
Jak/Src Pathway
BCL2 12.06 1.13 B-cell CLL/lymphoma 2 Bcl-2
BCL2L1 6.03 −7.04 BCL2-like 1 BCL-XL/S
NFkB Pathway
BCL2A1 1.50 2.33 BCL2-related protein A1 ACC-1/ACC-2
BIRC2 2.13 3.24 Baculoviral IAP repeat-containing 2 API1/HIAP2
BIRC3 1.06 −2.48 Baculoviral IAP repeat-containing 3 AIP1/API2
NAIP (BIRC1) 2.13 1.14 NLR family, apoptosis inhibitory protein BIRC1/NLRB1
TERT 12.06 1.13 Telomerase reverse transcriptase EST2/TCS1
P53 Pathway
BAX 3.01 −14.1 BCL2-associated X protein BCL2L4
CDKN1A 3.00 −2.47 Cyclin-dependent kinase inhibitor 1A (p21, Cip1) CAP20/CDKN1
Fas −1.33 −1.25 Fas (TNF receptor superfamily, member 6) ALPS1A/APO-1
GADD45A 5.99 2.26 Growth arrest and DNA-damage-inducible, alpha DDIT1/GADD45
IGFBP3 −14.95 −40 Insulin-like growth factor binding protein 3 BP-53/IBP3
MDM2 1.06 −4.93 Mdm2 p53 binding protein homolog (mouse) HDMX/hdm2
TP5313 4.28 −1.22 Tumor protein p53 inducible protein 3 PIG3
Stress Pathway
ATF2 3.01 −2.48 Activating transcription factor 2 CRE-BP1/CREB2
FOS 67.78 1.6 V-fos FBJ murine osteosarcoma viral oncogene homolog AP-1/C-FOS
HSF1 (tcf5) 4.25 1.15 Heat shock transcription factor 1 HSTF1
HSPB1 (hsp27) 4.27 −1.25 Heat shock 27 kDa protein 1 CMT2F/DKFZp586P1322
HSPCA (hsp90) 1.50 −3.48 Heat shock protein 90 kDa alpha (cytosolic), class A member 2 HSP90ALPHA/HSPCA
MYC 4.28 −3.56 V-myc myelocytomatosis viral oncogene homolog (avian) MRTL/bHLHe39
TP53 1.07 −1.75 Tumor protein p53 LFS1/TRP53
NFkB Pathway
IKBKB 2.11 −2.53 Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta IKK-beta/IKK2
IL1A 8.48 3.27 Interleukin 1, alpha IL-1A/IL1
IL2 11.72 1.17 Interleukin 2 IL-2/TCGF
IL8 1.53 −9.92 Interleukin 8 CXCL8/GCP-1
LTA (TNF beta) 11.75 1.13 Lymphotoxin alpha (TNF superfamily, member 1) LT/TNFB
NOS2A (iNOS) 1.42 −3.52 Nitric oxide synthase 2, inducible HEP-NOS/INOS
PECAM1 8.03 1.09 Platelet/endothelial cell adhesion molecule CD31/PECAM-1
TANK 5.70 −3.63 TRAF family member-associated NFKB activator I-TRAF/TRAF2
TNF 7.99 −1.75 Tumor necrosis factor (TNF superfamily, member 2) DIF/TNF-alpha
VCAM1 12.06 1.13 Vascular cell adhesion molecule 1 CD106/DKFZp779 G2333
NFAT Pathway
CD5 11.65 1.13 CD5 molecule LEU1/T1
FASLG (TNFSF6) 11.69 1.16 Fas ligand (TNF superfamily, member 6) APT1LG1/CD178
IL2 11.72 1.17 Interleukin 2 IL-2/TCGF
CREB Pathway
CYP19A1 11.27 1.13 Cytochrome P450, family 19, subfamily A, polypeptide 1 ARO/ARO1
EGR1 12.06 1.16 Early growth response 1 AT225/G0S30
FOS 67.78 1.6 V-fos FBJ murine osteosarcoma viral oncogene homolog AP-1/C-FOS
Jak-Stat pathway
CXCL9 11.14 1.13 Chemokine (C-X-C motif) ligand 9 CMK/Humig
IL4 11.33 1.13 Interleukin 4 BCGF-1/BCGF1
IL4R 1.51 −3.52 Interleukin 4 receptor CD124/IL4RA
MMP10 3.02 −1.76 Matrix metallopeptidase 10 (stromelysin 2) SL-2/STMY2
NOS2A (iNOS) 1.42 −3.52 Nitric oxide synthase 2, inducible HEP-NOS/INOS
Estrogen Pathway
BCL2 12.06 1.13 B-cell CLL/lymphoma 2 Bcl-2
BRCA1 8.50 1.1 Breast cancer 1, early onset BRCAI/BRCC1
GREB1 11.72 1.16 GREB1 protein KIAA0575
NRIP1 −1.32 −3.51 Nuclear receptor interacting protein 1 RIP140
Androgen Pathway
CDK2 8.55 −1.75 Cyclin-dependent kinase 2 p33(CDK2)
CDKN1A 3.00 −2.47 Cyclin-dependent kinase inhibitor 1A (p21, Cip1) CAP20/CDKN1
KLK2 11.41 1.16 Kallikrein-related peptidase 2 KLK2A2/hK2
TMEPAI −1.87 −1.74 Prostate transmembrane protein, androgen induced 1 STAG1/TMEPAI
Calcium and protein kinase C Pathway
CSF2 11.42 −1.84 Colony stimulating factor 2 (granulocyte-macrophage) GMCSF
FOS 67.78 1.6 V-fos FBJ murine osteosarcoma viral oncogene homolog AP-1/C-FOS
IL2 11.72 1.17 Interleukin 2 IL-2/TCGF
JUN 8.51 1.64 Jun oncogene AP-1/AP1
MYC 4.28 −3.56 V-myc myelocytomatosis viral oncogene homolog (avian) MRTL/bHLHe39
ODC1 8.54 1.65 Ornithine decarboxylase 1 ODC
PRKCA 5.62 1.13 Protein kinase C, alpha AAG6/PKC-alpha
PRKCE 2.92 −2.48 Protein kinase C, epsilon PKCE/nPKC-epsilon
TFRC −1.30 −1.74 Transferrin receptor (p90, CD71) CD71/TFR
Phospholipase C Pathway
BCL2 12.06 1.13 B-cell CLL/lymphoma 2 Bcl-2
EGR1 12.06 1.16 Early growth response 1 AT225/G0S30
FOS 67.78 1.6 V-fos FBJ murine osteosarcoma viral oncogene homolog AP-1/C-FOS
ICAM1 −2.68 −20.07 Intercellular adhesion molecule 1 BB2/CD54
JUN 8.51 1.64 Jun oncogene AP-1/AP1
NOS2A 1.42 −3.52 Nitric oxide synthase 2, inducible HEP-NOS/INOS
PTGS2 23.98 4.57 Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) COX-2/COX2
VCAM1 12.06 1.13 Vascular cell adhesion molecule 1 CD106/DKFZp779G2333
Insulin Pathway
CEBPB 2.90 3.3 CCAAT/enhancer binding protein (C/EBP), beta C/EBP-beta
FASN 4.26 1.14 Fatty acid synthase FAS/OA-519
GYS1 3.03 3.29 Glycogen synthase 1 (muscle) GSY/GYS
HK2 2.99 1.69 Hexokinase 2 DKFZp686M1669/HKII
LEP 12.06 1.13 Leptin OB/OBS
LDL Pathway
CCL2 8.79 1.13 Chemokine (C-C motif) ligand 2 GDCF-2/HC11
CSF2 11.42 −1.84 Colony stimulating factor 2 (granulocyte-macrophage) GMCSF
SELE 11.70 1.13 Selectin E CD62E/ELAM
SELPLG 12.06 1.13 Selectin P ligand CD162/CLA
VCAM1 12.06 1.13 Vascular cell adhesion molecule 1 CD106/DKFZp779 G2333
Retinoic acid Pathway
EN1 11.76 1.13 Engrailed homeobox1 Engrailed 1
HOXA1 12.06 1.13 Homeobox A1 BSAS/HOX1
RBP1 (CRBP1) 1.06 −1.74 Retinol binding protein 1, cellular CRABP-I/CRBP