Figure 4. The relative levels of vimentin and αB-crystallin in Human primary lens epithelial cells after RNA interference (RNAi), measured by western blot. The internal control is Glyceraldehyde 3-phosphate dehydrogenase (GAPDH; A). The expression of vimentin in different groups of human primary epithelial lens cells measured 48 h after lentiviral infection. The levels of Vimentin in the control group are considerably higher compared to shRNA-1, 2, 3 groups (p<0.05). There are no significant differences between the levels of Vimentin detected in the control group and the shRNA-scramble group (p>0.05, shRNA-scr with random RNA sequence). One-way analysis of variance (ANOVA) were applied to each treatment (n=3), and the standard error of the mean (SEM) was used in the graph (B). The expression of αB-crystallin in different groups of human primary epithelial lens cells measured 48 h after lentiviral infection. The levels of αB-crystallin in the control group were considerably lower compared to shRNA-1, 2, 3 groups (p<0.05). There were no significant differences between the levels of αB-crystallin detected in the control group and the shRNA-scramble group (p>0.05, shRNA-scr with random RNA sequence). One-way analysis of variance (ANOVA) was applied to each treatment (n=3),and the standard error of the mean (SEM) was used in the graph (C).