Figure 3. The relative RNA level of pigment epithelium-derived factor (PEDF) in human primary lens epithelial cells after RNA interference. Q-PCR was used to quantify the PEDF mRNA transcript 48 h after the infection. The relative PEDF mRNA expression level was reduced (p<0.01) in the sample 2 (shRNA1), sample 3 (shRNA2), and sample 4 (shRNA3) compared with control group. The mRNA transcripts were not significant differences (p>0.05) between control group and sample 1 (shRNA1 with random RNA sequence). The PEDF mRNA was significantly down regulated with RNAi in group 2, 3, and 4. We used One-way analysis of variance (ANOVA); each experiment was repeated 3 times (n=3).