Table 2 of Wang, Mol Vis 2010; 16:1146-1153. Table 2 of Wang, Mol Vis 2010; 16:1146-1153.
Table 2. Primers for amplifying and sequencing PAX3 genomic fragments.
Exon |
Forward Primers(5′-3′) |
Reverse Primers(5′-3′) |
Product size (bp) |
Annealing temperature (°C) |
|---|---|---|---|---|
| PAX3-ex1 | TCACCACAGGAGGAGACTCA | GAGGCCCTCCCTTACCTTC | 472 | 60 |
| PAX3-ex2 | TACGTGCTGCTGTTCTTTGC | TTACGCACCTTCACAAACCTC | 442 | 60 |
| PAX3-ex3 | TCTGGTCTGCCCCTTTCTAA | ATTGGGGTGATTACGTCTGG | 388 | 60 |
| PAX3-ex4 | GCTGGAGAAGGATGAGGATG | CTCCAAGTGACCCAGCAAGT | 351 | 60 |
| PAX3-ex5 | TGTCTTGCAGTCGGAGAGAG | GGTGGACTTCTGTGTGTCGT | 492 | 60 |
| PAX3-ex6 | AATTCGCCCAAACAACACA | CAGAGAAATCGCCTGGAAGT | 368 | 60 |
| PAX3-ex7 | TGGCGATGAACTTTTGCAC | GGGTGGAGAGAAAGGAAACC | 451 | 60 |
| PAX3-ex8 | TCGTCGGGCATGATGTAATA | AGGAGAAATTGCCCCCTAAA | 359 | 60 |
| PAX3-ex9 | GAATTGTCCCAGCATGACCT | TGCTCCAGGTCTTCCTCTTC | 311 | 62 |
| PAX3-ex10a | ACTGGCCCTGTTTCTGGTCT | TGGCAAACATCACTGCACTC | 943 | 60 |
| PAX3-ex10b | CCAGTTCACATTTATTTGG | CTCATAGAAAGGGTCCAC | 887 | 60 |