Molecular Vision: Cai, Mol Vis 2010; 16:1141-1145. Table 1

Table 1. Primers used for polymerase chain reaction amplification and sequencing of PAX6.

			Annealing temperature °C
Exon	Sense primer(5’-3’)	Antisense primer (5’-3’)	I	II	Product size (bp)
5	GGCTGGTGGTCCTGTTGTCCTT	CGAGCCCGAAGTCCCAGAAAG	61.5	55	483
6, 7	AAGCAAGGTCAGCACAAAAATAAATT	GGAGGAGGTAAAGAGGAGAGAGCATT	60.5	54	648
8	TAAGGTTGTGGGTGAGCTGAGATG	GGGAGAGTAGGGGACAGGCAAAGG	61.5	55	315
9	TTTGGTGAGGCTGTCGGGATATAAT	TGCCCAGAGAAATAAAAAGACAGAAA	61.5	55	415
10	TTGGTTGGAGGTAATGGGAGTGG	TGGCAGCAGAGCATTTAGCAGAC	61.5	55	334
11, 12	GGGGCTGGGCTCGACGTAG	GCCACCACCAGCCGCACTTA	65	60	438
13	GGGGCTGTGGCTGTGTGATGT	CCCCAGGGACAAGGAAAGCAA	61.5	55	333
14	CCAAACATGCAAACAAACAGAGGA	TTCCAACTGATATCGTGCCTTCTG	61.5	55	570