Figure 5. PPARγ (green) and nuclei (DAPI, red) staining during meibomian gland morphogenesis. At E18.5 (A) and P0 (B), PPARγ staining of the epithelial placode was negative. At P3 (C), PPARγ was first detected in the lumen of the developing gland and increased progressively as the gland continued to develop at P5 (D) and P8 (E). At P15 (F), PPARγ staining was limited to the acini (arrows) or the terminal regions of the branching ductules (the asterisk shows a well developed central duct at P15).