Figure 3 of Hjelmeland, Mol Vis 2010; 16:1108-1121.

Figure 3. Laser scanning cytometer schematic diagram. The laser scanning cytometer consists of an optics unit that generates the laser scanning beam, an upright epifluorescence microscope with a motorized stage to allow generation of sample scan images, and a computer to acquire and analyze scan data using WinCyte software. Fluorescence is excited by laser sources consisting of an argon laser (blue light; 488 nm), a helium–neon laser (HeNe; red light; 633 nm), and a violet diode laser (405 nm) [35].