Figure 11. Double immunolabeling for young and old retinal pigment epithelium (RPE). Detection of retinal pigment epithelium cells double immunolabeled for Mn-superoxide dismutase (MnSOD) and retinal pigment epithelium-specific protein 65 kDa (RPE65) proteins in sections of human eyes at 14 months and 64 years by laser scanning cytometry. Formalin-fixed paraffin-embedded sections were prepared and processed for laser scanning cytometry from a 14-month-old male and a 64-year-old female eye. Panel A shows data for the 14-month-old eye double immunolabeled for MnSOD and RPE65 (6 μg/ml mouse monoclonal antibovine RPE65, 4 μg/ml goat anti-SOD2 primary antibodies and 4 μg/ml Alexa Fluor 488-conjugated donkey antimouse IgG and 40 μg/ml Alexa Fluor 647-conjugated donkey antigoat IgG secondary antibodies). Panel B shows a similar analysis of a section from a 64-year-old female eye.