Table 2 of Ren, Mol Vis 2010; 16:1076-1086. Table 2 of Ren, Mol Vis 2010; 16:1076-1086.
Table 2. Characteristics of primers, probes, and PCR efficiencies of the housekeeping and target genes used in this study.
Gene |
Oligo |
Sequence |
Amplicon (bps) |
PCR efficiency |
|---|---|---|---|---|
| GAPDH NM_008084 | F | TGTGTCCGTCGTGGATCTGA | 77 | 91.3% |
| R | CCTGCTTCACCACCTTCTTGA | |||
| P | CCGCCTGGAGAAACCTGCCAAGTATG | |||
| LDHA NM_010699 | F | ATCCCATTTCCACCATGATT | 183 | 86% |
| R | ACTGCAGCTCCTTCTGGATT | |||
| P | CAGGCGGGCCTCTTCCTCAG | |||
| ACTB NM_007393 | F | GCAAGCAGGAGTACGATGAG | 148 | 92.7% |
| R | CCATGCCAATGTTGTCTCTT | |||
| P | TCCATCGTGCACCGCAAGTG | |||
| UBC NM_019639 | F | ACCAGCAGAGGCTGATCTTT | 110 | 88.7% |
| R | ACCTCTGAGGCGAAGGACTA | |||
| P | CTGGAAGATGGCCGCACCCT | |||
| PPIA NM_008907 | F | AATGCTGGACCAAACACAAA | 117 | 100.3% |
| R | TTCCACAATGTTCATGCCTT | |||
| P | TGCTTGCCATCCAGCCATTCA | |||
| TBP1 NM_013684 | F | ATCAACATCTCAGCAACCCA | 187 | 97.1% |
| R | TTGAAGCTGCGGTACAATTC | |||
| P | ACCACTGCACCGTTGCCAGG | |||
| HPRT1 NM_013556 | F | GGCCAGACTTTGTTGGATTT | 155 | 95.1% |
| R | CAGATTCAACTTGCGCTCAT | |||
| P | TGACACAAACGTGATTCAAATCCCTG | |||
| RPL5 NM_016980 | F | GGAAGCACATCATGGGTCAGA | 70 | 91.8% |
| R | TACGCATCTTCATCTTCCTCCATT | |||
| P | TGTGGCAGACTACATGCGCTACC | |||
| Tkt NM_009388 | F | GACAGTGCCCTTCTGCAGTACTT | 65 | 101.1% |
| R | CCATGCGAATCTGGTCGAA | |||
| P | CGCGGCCTTCTTCACACGGG |