Figure 3. Changes in glutathione level
during lens culture. Bovine lenses were incubated in a final volume of
40 ml in a GSH/Gly-Gly medium (see Methods) alone (circles), or in the
presence of 10 mM serine/borate (squares) or 10 mM serine/borate and
0.5 mM Cys-Gly (triangles). Closed and open symbols refer to the
extra-lenticular level of total glutathione and GSSG, respectively.
Inset: bars refer to total glutathione intra-lenticular level measured
after 24 h of incubation in the following conditions: a: standard
medium; b: GSH/Gly-Gly medium; c: GSH/Gly-Gly/SB medium; d:
GSH/Gly-Gly/SB medium supplemented with Cys-Gly. Statistical analysis
was performed comparing the values measured under different conditions
at the same time of incubation. (#): p<0.05 versus GSH/Gly-Gly
medium, (###): p<0.001 versus GSH/Gly-Gly medium, (*): p<0.05
versus GSH/Gly-Gly medium supplemented with Cys-Gly, and
(***):<0.001 versus GSH/Gly-Gly medium supplemented with Cys-Gly.
Values are reported as the mean of at least five different experiments;
the error bars represent the standard error of the mean.