Figure 3. Sequence analysis of the
unaffected members and affected members in the family with congenital
cataract and microcornea. A: The partial sequence of CRYBA4
in a normal individual is displayed. B: The corresponding
nucleotide sequence of CRYBA4 in the proband is shown.
Sequencing results showed a substitution of G→T in CRYBA4
(c.225G>T), which led to replacement of glycine by tryptophan at
codon 64(G64W) in crystallin, beta A4 protein.