Figure 3. Sequence analysis of the unaffected members and affected members in the family with congenital cataract and microcornea. A: The partial sequence of CRYBA4 in a normal individual is displayed. B: The corresponding nucleotide sequence of CRYBA4 in the proband is shown. Sequencing results showed a substitution of G→T in CRYBA4 (c.225G>T), which led to replacement of glycine by tryptophan at codon 64(G64W) in crystallin, beta A4 protein.