Figure 3. The corrective effect of 4-PBA on G165fsX8-expressing cells. A-D: 4-PBA corrected the mislocalization of G165fs CRYGD as shown by confocal immunofluorescence. A: Untreated WT cells and B: WT cells treated with 1 mM PBA for two days. C: Untreated G165fs CRYGD cells and D: G165fs CRYGD cells treated with 1 mM PBA for two days. E: 4-PBA reduced apoptosis of mutant cells with nuclear fragmentation. The asterisk indicates a p<0.05 by independent Student’s t-test. F: Semi-quantitative RT–PCR analysis showed specific upregulation of Hsp70 in G165fs CRYGD cells treated with 4-PBA (p<0.05, independent Student’s t-test). No significant change was found for Hsp90.