Figure 2. 4-PBA improved the solubility of various cataract-causing CRYGD mutants expressing in COS-7 cells. A: western blotting of FLAG and housekeeping proteins in Tx soluble and insoluble fractions from cells expressing WT, R15C, R15S, P24T, R61C, or G165fsX8 CRYGD treated (right) or not (left) with 1 mM 4-PBA for two days. B: Band densitometry of FLAG-tagged CRYGD normalized with housekeeping proteins in cells from A. Asterisk indicated a p<0.05 by independent Student’s t-test.