Figure 1. Chemical chaperone 4-PBA improved Tx solubility of G165fsX8 CRYGD expressing in COS-7 cells. Cells were treated with 4-PBA (0 to 3 mM) for two days followed by western blotting for FLAG-tagged CRYGD and band densitometry (represented by histogram). A: Tx insoluble fractions. B: Tx soluble fractions. β-Actin and GAPDH were the housekeeping proteins of Tx-insoluble and soluble fractions, respectively. Asterisks indicate a p<0.05 by independent Student’s t-test. C: Treatment of cells with TMAO (0 to 300 mM) for two days followed by Tx solubility test for FLAG-tagged CRYGD expression. D: Band densitometry analysis showed that TMAO did not affect the mutant protein solubility.