Table 1 of Kashiwagi, Mol Vis 2010; 16:970-982. Table 1 of Kashiwagi, Mol Vis 2010; 16:970-982.
Table 1. PCR primers and PCR schedules.
| Gene | Nucleotide (5′-3′) | Accession number | Product (bp) | Anneaing (°C)/cycles |
|---|---|---|---|---|
| β-Actin | F: CCCATGCCATCCTGCGTCTG | NM_001101 | 573 | 60 °C/20 cycles |
| R: CGTCATACTCCTGCTTGCTG | ||||
| PAX6 | F: ATGGTTTTCTAATCGAAGGG | NM_000280 | 149 | 58 °C/33 cycles |
| R: CGGTGTGGTGGGTTGTGGAAT | ||||
| RHO | F: CATCGAGCGGTACGTGGTGGTGTG | NM_000539 | 577 | 65 °C/33 cycles |
| R: GCCGCAGCAGATGGTGGTGAGC | ||||
| P21 | F: CTCCAAGAGGAAGCCCTAATCC | NM_000389 | 535 | 60 °C/25 cycles |
| R: TTTGATGATGCCCCCACTCG | ||||
| N-myc | F: GACCACAAGGCCCTCAGTAC | NM_005378 | 240 | 60 °C/25 cycles |
| R: GTGGATGGGAAGGCATCGTT | ||||
| PAX6-P0 promoter | F: GGTACCTTTTCCTATGAGGGCAAGAC | NG_008679 | 5315 | 60 °C/30 cycles |
| R: GCTAGCTCCCTCAGTAACTCGCTTCC | ||||
| PAX6-P1 promoter | F:GGTACCCTGCAAAAAGAGAGACGTTTGGGGC | NG_008679 | 3796 | 60 °C/30 cycles |
| R: GCTAGCCTTTATGAGGCATCCTTTCTGG | ||||
| PAX6-Pa promoter | F: GGTACCCAGAGCCAGCATGCAGAACA | NG_008679 | 3628 | 60 °C/30 cycles |
| R: GCTAGCCTGATTCACTCCGCTGTGAC | ||||
| PAX6 (exon 11–13) | F: AACAGACACAGCCCTCACAAACA | NM_000280 | 275 | 55 °C/35 cycles |
| R: CGGGAACTTGAACTGGAACTGAC | ||||
| PAX6 (exon 3–6) | F: GGAAGACTTTAACTAGGGGC | NM_000280 | 416 | 58 °C/33 cycles |
| R: ATGGACGGGCACTCCCGCTT |